Motility Evaluation of Human Spermatozoa

Motility evaluation of human spermatozoa

In this contribution we investigate the applicability of photon correlation techniques as a clinical tool for the evaluation of motility parameters of undiluted human sperm. Measurements on semen samples, selected visually for 'good' motility, yielded reproducible estimates of the fraction motile spermatozoa and the mean speed of the motile cells.

Motility of spermatozoa at surfaces.

The hydrodynamic basis for the accumulation of spermatozoa at surfaces has been investigated. The general conclusion is that when spermatozoa arrive at a surface, they will remain there if the vector of the time-averaged thrust is directed towards that surface. This can arise in two basic ways. First, consider spermatozoa that maintain a three-dimensional waveform and roll (spin) as they progre...

Human mtDNA haplogroups associated with high or reduced spermatozoa motility.

A variety of mtDNA mutations responsible for human diseases have been associated with molecular defects in the OXPHOS system. It has been proposed that mtDNA genetic alterations can also be responsible for sperm dysfunction. In addition, it was suggested that if sperm dysfunction is the main phenotypic consequence, these mutations could be fixed as stable mtDNA variants, because mtDNA is matern...

Formation of Hydrogen Peroxide by Chilled Goat Spermatozoa and the Effects of Dead Spermatozoa on Motility Characteristics

In this study, the formation of hydrogen peroxide (H2O2) by chilled goat spermatozoa was measured. Furthermore, the effects of dead spermatozoa on motility characteristics were studied. Fresh collected ejaculates from five Shami bucks were centrifuged and virtually all seminal plasma was removed. A part of the collected spermatozoa was killed by two ways: the first by repe...

Evaluation of Serial Thawing-Refreezing on Human Spermatozoa Resistance Using Cryovials and Straws